Antimicrobial substance from a human Lactobacillus strain

作者：M Silva, Nilda V. Jacobus, Carl F. Deneke, Sherwood L. Gorbach · 发表于：Antimicrobial Agents and Chemotherapy · 年份：1987 · DOI：10.1128/aac.31.8.1231 · 被引用次数：562 · 研究领域：Probiotics and Fermented Foods、Microbial Metabolites in Food Biotechnology、Enzyme Production and Characterization

Lactobacillus sp. strain GG, which was isolated from the feces of a normal person, produced a substance with potent inhibitory activity against a wide range of bacterial species. It inhibited anaerobic bacteria (Clostridium spp., Bacteroides spp., Bifidobacterium spp.), members of the family Enterobacteriaceae, Pseudomonas spp. Staphylococcus spp., and Streptococcus spp., as demonstrated by a microbiological assay; however, it did not inhibit other lactobacilli. The inhibitory activity occurred between pH 3 and 5 and was heat stable. Bactericidal activity against Escherichia coli was demonstrated at a dilution of 1:128. The inhibitory substance was distinct from lactic and acetic acids. It had a low molecular weight (less than 1,000) and was soluble in acetone-water (10:1). Because of these characteristics, the inhibitory material could not be considered a bacteriocin; it most closely resembled a microcin, which has been associated previously with members of the family Enterobacteriaceae.