RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA

作者：Charles L. Christian · 发表于：The Journal of Experimental Medicine · 年份：1963 · DOI：10.1084/jem.118.5.827 · 被引用次数：54 · 研究领域：Protein purification and stability、Clostridium difficile and Clostridium perfringens research、Glycosylation and Glycoproteins Research

The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectrophoretic analysis and analytical ultracentrifugation. Certain RFLS sera demonstrated selective reactivity with one or more rabbit isohemagglutinin antibodies. Pepsin treatment of rabbit gamma-globulin removed or greatly diminishes its capacity to react with the RFLS.