LC‐MS/MS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study

作者：Xiaolin Wang, Dan Zhang, Man Liu, Hongna Zhao, Aihua Du, Lingjie Meng, Huichen Liu · 发表于：Biomedical Chromatography · 年份：2013 · DOI：10.1002/bmc.3008 · 被引用次数：24 · 研究领域：Circadian rhythm and melatonin、Biochemical Analysis and Sensing Techniques、Tea Polyphenols and Effects

A sensitive and selective LC-MS/MS method for the determination of agomelatine in human plasma was developed and validated. After simple liquid-liquid extraction, the analytes were separated on a Zorbax SB-C18 column (150 × 2.1 mm i.d., 5 µm) with an isocratic mobile phase consisting of 5 mm ammonium acetate solution (containing 0.1% formic acid) and methanol (30:70, v/v) at a flow-rate of 0.3 mL/min. The MS acquisition was performed in multiple reaction monitoring mode with a positive electrospray ionization source. The mass transitions monitored were m/z 244.1 → 185.3 and m/z 285.2 → 193.2 for agomelatine and internal standard, respectively. The methods were validated for selectivity, carry-over, matrix effects, calibration curves, accuracy and precision, extraction recoveries, dilution integrity and stability. The validated method was successfully applied to a pharmacokinetic study of agomelatine in Chinese volunteers following a single oral dose of 25 mg agomelatine tablet.