Ion Mobility Analysis of Molecular Dynamics

作者：Thomas Wyttenbach, Nicholas A. Pierson, David E. Clemmer, Michael T. Bowers · 发表于：Annual Review of Physical Chemistry · 年份：2013 · DOI：10.1146/annurev-physchem-040513-103644 · 被引用次数：193 · 研究领域：Mass Spectrometry Techniques and Applications、Analytical Chemistry and Chromatography、Advanced Proteomics Techniques and Applications

The combination of mass spectrometry and ion mobility spectrometry (IMS) employing a temperature-variable drift cell or a drift tube divided into sections to make IMS-IMS experiments possible allows information to be obtained about the molecular dynamics of polyatomic ions in the absence of a solvent. The experiments allow the investigation of structural changes of both activated and native ion populations on a timescale of 1-100 ms. Five different systems representing small and large, polar and nonpolar molecules, as well as noncovalent assemblies, are discussed in detail: a dinucleotide, a sodiated polyethylene glycol chain, the peptide bradykinin, the protein ubiquitin, and two types of peptide oligomers. Barriers to conformational interconversion can be obtained in favorable cases. In other cases, solution-like native structures can be observed, but care must be taken in the experimental protocols. The power of theoretical modeling is demonstrated.