Diagnosing the miR-141 prostate cancer biomarker using nucleic acid-functionalized CdSe/ZnS QDs and telomerase

作者：Amily Fang‐Ju Jou, Chunhua Lü, Yen‐Chuan Ou, Shian‐Shiang Wang, Shih‐Lan Hsu, Itamar Willner, Ja‐an Annie Ho · 发表于：Chemical Science · 年份：2014 · DOI：10.1039/c4sc02104e · 被引用次数：129 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、MicroRNA in disease regulation

M. The first step of the sensing platform is non-selective toward miR-141 and the resulting fluorescence may be considered only as an indicator for the existence of miR-141. The second step in the sensing protocol, involving telomerase, provides a selective chemiluminescence signal for the existence of miR-141. The two-step sensing platform is implemented for the analysis of miR-141 in serum samples from healthy individuals and prostate cancer carriers. Impressive discrimination between healthy individuals and prostate cancer carriers is demonstrated.