Antifreeze Protein Produced Endogenously in Winter Rye Leaves

作者：Marilyn Griffith, Paul Ala, Daniel S.C. Yang, Wai‐Ching Hon, Barbara A. Moffatt · 发表于：PLANT PHYSIOLOGY · 年份：1992 · DOI：10.1104/pp.100.2.593 · 被引用次数：276 · 研究领域：Magnetic and Electromagnetic Effects、Freezing and Crystallization Processes、Physiological and biochemical adaptations

After cold acclimation, winter rye (Secale cereale L.) is able to withstand the formation of extracellular ice at freezing temperatures. We now show, for the first time, that cold-acclimated winter rye plants contain endogenously produced antifreeze protein. The protein was extracted from the apoplast of winter rye leaves, where ice forms during freezing. After partial purification, the protein was identified as antifreeze protein because it modified the normal growth pattern of ice crystals and depressed the freezing temperature of water noncolligatively.