Quantitative Genetic Bases of Anthocyanin Variation in Grape ( Vitis vinifera L. ssp. sativa ) Berry: A Quantitative Trait Locus to Quantitative Trait Nucleotide Integrated Study

作者：Alexandre Fournier‐Level, Loïc Le Cunff, Camila Gomez, Agnès Doligez, Agnès Ageorges, Catherine Roux, Yves Bertrand, Jean‐Marc Souquet, Véronique Cheynier, Patrice This · 发表于：Genetics · 年份：2009 · DOI：10.1534/genetics.109.103929 · 被引用次数：198 · 研究领域：Horticultural and Viticultural Research、Fermentation and Sensory Analysis、Plant Gene Expression Analysis

The combination of QTL mapping studies of synthetic lines and association mapping studies of natural diversity represents an opportunity to throw light on the genetically based variation of quantitative traits. With the positional information provided through quantitative trait locus (QTL) mapping, which often leads to wide intervals encompassing numerous genes, it is now feasible to directly target candidate genes that are likely to be responsible for the observed variation in completely sequenced genomes and to test their effects through association genetics. This approach was performed in grape, a newly sequenced genome, to decipher the genetic architecture of anthocyanin content. Grapes may be either white or colored, ranging from the lightest pink to the darkest purple tones according to the amount of anthocyanin accumulated in the berry skin, which is a crucial trait for both wine quality and human nutrition. Although the determinism of the white phenotype has been fully identified, the genetic bases of the quantitative variation of anthocyanin content in berry skin remain unclear. A single QTL responsible for up to 62% of the variation in the anthocyanin content was mapped on a Syrah x Grenache F(1) pseudo-testcross. Among the 68 unigenes identified in the grape genome within the QTL interval, a cluster of four Myb-type genes was selected on the basis of physiological evidence (VvMybA1, VvMybA2, VvMybA3, and VvMybA4). From a core collection of natural resources (141 in...