Evaluation of the genetic diversity and population structure of Chinese indigenous horse breeds using 27 microsatellite markers

作者：Yinghui Ling, Y. H., W. J. Guan, Yunlian Cheng, Yi-Zhang Wang, J. L. Han, L. Mang, Qingbo Zhao, Xuelian He, Yuan-xu Pu, B. L. Fu · 发表于：Animal Genetics · 年份：2010 · DOI：10.1111/j.1365-2052.2010.02067.x · 被引用次数：44 · 研究领域：Genetic diversity and population structure、Genetic and phenotypic traits in livestock、Genomics and Phylogenetic Studies

We determined the genetic diversity and evolutionary relationships among 26 Chinese indigenous horse breeds and two introduced horse breeds by genotyping these animals for 27 microsatellite loci. The 26 Chinese horse breeds come from 12 different provinces. Two introduced horse breeds were the Mongolia B Horse from Mongolia and the Thoroughbred Horse from the UK. A total of 330 alleles were detected, and the expected heterozygosity ranged from 0.719 (Elenchuns) to 0.780 (Dali). The mean number of alleles among the horse breeds ranged from 6.74 (Hequ) to 8.81 (Debao). Although there were abundant genetic variations found, the genetic differentiation was low between the Chinese horses, which displayed only 2.4% of the total genetic variance among the different breeds. However, genetic differentiation (pairwise FST) among Chinese horses, although moderate, was still apparent and varied from 0.001 for the Guizou-Luoping pair to 0.064 for the Jingjiang-Elenchuns pair. The genetic differentiation patterns and genetic relationships among Chinese horse breeds were also consistent with their geographical distribution. The Thoroughbred and Mongolia B breeds could be discerned as two distinct breeds, but the Mongolia B horse in particular suffered genetic admixture with Chinese horses. The Chinese breeds could be divided into five major groups, i.e. the south or along the Yangtze river group (Bose, Debao, Wenshan, Lichuan, Jianchang, Guizhou, Luoping, Jinjiang and Dali), the Qinghai-Tib...