ENZYMIC DEPHOSPHORYLATION OF PEPSIN AND PEPSINOGEN

作者：Gertrude E. Perlmann · 发表于：The Journal of General Physiology · 年份：1958 · DOI：10.1085/jgp.41.3.441 · 被引用次数：23 · 研究领域：Diet, Metabolism, and Disease、Enzyme Catalysis and Immobilization、Metabolism, Diabetes, and Cancer

It has been shown by the work presented in this paper that it is possible to dephosphorylate enzymically pepsin and pepsinogen with a variety of phosphatases. With the aid of a phosphodiesterase and the prostate phosphatase it has been established that the phosphorus in the two proteins is present as a diester and connects two sites of the peptide chain in a cyclic configuration. Removal of the phosphorus does not affect the proteolytic activity against hemoglobin or the synthetic substrate acetyl-L-phenylalanyl diiodotryosine, nor the pepsinogen pepsin transformation. However, an increase of the autodigestion of pepsin is observed.