Identification of (E)‐4‐hydroxy‐3‐methyl‐but‐2‐enyl pyrophosphate as a major activator for human γδ T cells in Escherichia coli

作者：Martin Hintz, Armin Reichenberg, Boran Altincicek, U. Bahr, Ruth M. Gschwind, Ann‐Kristin Kollas, Ewald Beck, Jochen Wiesner, Matthias Eberl, Hassan Jomaa · 发表于：FEBS Letters · 年份：2001 · DOI：10.1016/s0014-5793(01)03191-x · 被引用次数：361 · 研究领域：Plant biochemistry and biosynthesis、Natural product bioactivities and synthesis、Microbial Natural Products and Biosynthesis

The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli. In lytB-deficient mutants, a highly immunogenic compound accumulates significantly, compared to wild-type E. coli, but is apparently absent in gcpE-deficient mutants. Here, this compound was purified from E. coli DeltalytB mutants by preparative anion exchange chromatography, and identified by mass spectrometry, (1)H, (13)C and (31)P NMR spectroscopy, and NOESY analysis as (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP). HMB-PP is 10(4) times more potent in activating human Vgamma9/Vdelta2 T cells than isopentenyl pyrophosphate.