Butein is a novel anti-adipogenic compound

作者：No‐Joon Song, Hyang-Jin Yoon, Ki Hyun Kim, So-Ra Jung, Woo-seok Jang, Chorong Seo, Young Min Lee, Dae‐Hyuk Kweon, Joung‐Woo Hong, Jeong-Soo Lee, Ki‐Moon Park, Ki-Moon Park, Kang Ro Lee, Kye Won Park, Kye Won Park · 发表于：Journal of Lipid Research · 年份：2013 · DOI：10.1194/jlr.m035576 · 被引用次数：74 · 研究领域：Cytokine Signaling Pathways and Interactions、Adipokines, Inflammation, and Metabolic Diseases、NF-κB Signaling Pathways

Rhus verniciflua Stokes (RVS) has been used as a traditional herbal medicine for its various biological activities including anti-adipogenic effects. Activity-guided separation led to the identification of the anti-adipogenic functions of butein. Butein, a novel anti-adipogenic compound, robustly suppressed lipid accumulation and inhibited expression of adipogenic markers. Molecular studies showed that activated transforming growth factor-β (TGF-β) and suppressed signal transducer and activator of transcription 3 (STAT3) signaling pathways were mediated by butein. Analysis of the temporal expression profiles suggests that TGF-β signaling precedes the STAT3 in the butein-mediated anti-adipogenic cascade. Small interfering RNA-mediated silencing of STAT3 or SMAD2/3 blunted the inhibitory effects of butein on adipogenesis indicating that an interaction between two signaling pathways is required for the action of butein. Upon butein treatments, stimulation of TGF-β signaling was still preserved in STAT3 silenced cells, whereas regulation of STAT3 signaling by butein was significantly impaired in SMAD2/3 silenced cells, further showing that TGF-β acts upstream of STAT3 in the butein-mediated anti-adipogenesis. Taken together, the present study shows that butein, a novel anti-adipogenic compound from RVS, inhibits adipocyte differentiation through the TGF-β pathway followed by STAT3 and peroxisome proliferator-activated receptor γ signaling, further implicating potential roles of b...