Distribution of human papillomavirus (HPV) genotypes and bacterial vaginosis presence in cervical samples from Paraguayan indigenous

作者：Pamela Mongelós, Laura Mendoza, Isabel Rodriguez-Riveros, Amalia Castro, Graciela Giménez, Patricia Araujo, Malvina Páez, Wilberto Castro, Jorge Basiletti, Joaquín V. González, Gloria Echagüe, Valentina Diaz, Florentina Laspina, Santiago Ever, Ramón Marecos, Gerardo Deluca, Marı́a Alejandra Picconi · 发表于：International Journal of Infectious Diseases · 年份：2015 · DOI：10.1016/j.ijid.2015.08.007 · 被引用次数：27 · 研究领域：Cervical Cancer and HPV Research、Reproductive tract infections research、Women's cancer prevention and management

OBJECTIVE: To determine the frequency of human papillomavirus (HPV) types and to assess bacterial vaginosis (BV) possible associations with cervical infections in indigenous Paraguayan women of the Department of Presidente Hayes. METHODS: This study included 181 sexually active women without cervical lesions. HPV typing was performed by polymerase chain reaction with primers PGMY 09/11 followed by reverse line hybridization. BV was diagnosed by the Nugent criteria using the results from a Gram stain smear. RESULTS: Sixteen percent of women were positive for at least one high risk HPV type (HR-HPV). The most frequent genotypes were HPV 16 (4.4%), followed by HPV 58 (3.3%), HPV 45 (3.3%), HPV 53 (2.8%) and HPV 11 (2.8%). A significant association between HR-HPV and BV was observed (p=0.01). In addition, women with BV had a higher frequency of Chlamydia trachomatis (p=0.0007), Trichomonas vaginalis (p=0.00009), Mycoplasma hominis (p=0.001). CONCLUSIONS: A large variety of HPV genotypes was detected and showed a slightly different pattern from previous studies on urban women in Paraguay, with the predominance of HR-HPV. Furthermore, the information of co-infections involved in BV could be useful for the improvement of national prevention programs, as well as for laboratory surveillance of these genital infections.