Serum regulation of the estrogen responsiveness of the human breast cancer cell line MCF-7.

作者：Martin Pagé, John K. Field, N. P. Everett, Chris D. Green · 发表于：PubMed · 年份：1983 · 被引用次数：103 · 研究领域：Estrogen and related hormone effects、Retinoids in leukemia and cellular processes、Bioactive Compounds and Antitumor Agents

Initially after receiving MCF-7 cells, we were able to confirm their estrogen responsiveness. We observed significant increases in thymidine incorporation, in thymidine kinase activity, and in cell numbers in response to 10(-8) M estradiol. Subsequently, however, the cells failed to show a response to estradiol. A growth response to estradiol could be restored by increasing the serum concentration in the medium. Cells grown in 15% serum (calf or human) responded to estradiol with increased rates of growth and thymidine incorporation and increased activities of thymidine kinase and DNA polymerase. We suggest that there is present in serum a "factor" which can influence the expression of a growth response to estradiol.