The ArcB Sensor Kinase of Escherichia coli Autophosphorylates by an Intramolecular Reaction

作者：Gabriela R. Peña-Sandoval, Dimitris Georgellis · 发表于：Journal of Bacteriology · 年份：2010 · DOI：10.1128/jb.01401-09 · 被引用次数：49 · 研究领域：Endoplasmic Reticulum Stress and Disease、Bacterial Genetics and Biotechnology、Protein Structure and Dynamics

The Arc two-component system, comprising the ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous genes in response to the respiratory conditions of growth. ArcB is a tripartite histidine kinase whose activity is regulated by the oxidation of two cytosol-located redox-active cysteine residues that participate in intermolecular disulfide bond formation. Here we show that ArcB autophosphorylates through an intramolecular reaction which diverges from the usually envisaged intermolecular autophosphorylation of homodimeric histidine kinases.