Reference Values of Fetal Erythrocytes in Maternal Blood During Pregnancy Established Using Flow Cytometry

作者：Harry de Wit, Karin C. Nabbe, Jurgen Kooren, Henk J Adriaansen, Elianne A. Roelandse-Koop, Joost H. N. Schuitemaker, Johannes J.M.L. Hoffmann · 发表于：American Journal of Clinical Pathology · 年份：2011 · DOI：10.1309/ajcphl3vxy0vmlxl · 被引用次数：23 · 研究领域：Pregnancy and preeclampsia studies、Erythrocyte Function and Pathophysiology、Hemoglobinopathies and Related Disorders

The aim of our study was to assess the fetal RBC count in maternal blood during uncomplicated pregnancies from 26 weeks onward. We used a flow cytometric method specifically designed for use in a routine hematology analyzer. Pregnant women were recruited through midwives. The participating laboratories used the FMH QuikQuant method (Trillium Diagnostics, Brewer, ME) in a CELL-DYN Sapphire hematology analyzer (Abbott Diagnostics, Santa Clara, CA). The method is based on a monoclonal antibody to hemoglobin F. Flow cytometric data were analyzed by 2 independent observers. The 95th percentile reference range was estimated according to Clinical and Laboratory Standards Institute guidelines. A total of 236 samples were statistically analyzed. Gestational ages ranged from 21.6 to 41 weeks (mean, 32.0 weeks), and the fetal RBC count in maternal blood ranged from 0.00% to 0.50% (median, 0.025%). The fetal RBC count in maternal blood shows no correlation with gestational age. The established reference range during normal pregnancy is less than 0.125%.