Controlled encapsulation of single-cells into monodisperse picolitre drops

作者：Jon F., Dino Di Carlo, Katherine J. Humphry, Sarah Köster, Daniel Irimia, David A. Weitz, Mehmet Toner · 发表于：Lab on a Chip · 年份：2008 · DOI：10.1039/b805456h · 被引用次数：508 · 研究领域：Innovative Microfluidic and Catalytic Techniques Innovation、Microfluidic and Bio-sensing Technologies、Electrowetting and Microfluidic Technologies

Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation.