Macrophages induce differentiation of plasma cells through CXCL10/IP-10

作者：Wei Xu, HyeMee Joo, Sandra Clayton, Mélissa Dullaers, Marie-Cecile Herve, Derek Blankenship, M. Teresa de la Morena, Robert Balderas, Capucine Pïcard, Jean‐Laurent Casanova, Virginia Pascual, SangKon Oh, Jacques Banchereau · 发表于：The Journal of Experimental Medicine · 年份：2012 · DOI：10.1084/jem.20112142 · 被引用次数：101 · 研究领域：Immune Cell Function and Interaction、T-cell and B-cell Immunology、Immunotherapy and Immune Responses

In tonsils, CD138(+) plasma cells (PCs) are surrounded by CD163(+) resident macrophages (Ms). We show here that human Ms (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138(+)CD38(++) PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Ms is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10-deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Ms and B cells, in which IP-10 acts as a PC differentiation factor.