The high copper tolerance of Candida albicans is mediated by a P-type ATPase

作者：Ziva Weissman, Israela Berdicevsky, Benzion Cavari, Daniel Kornitzer · 发表于：Proceedings of the National Academy of Sciences · 年份：2000 · DOI：10.1073/pnas.97.7.3520 · 被引用次数：154 · 研究领域：Trace Elements in Health、Heavy Metal Exposure and Toxicity、Corrosion Behavior and Inhibition

The pathogenic yeast Candida albicans has higher resistance than the baker's yeast Saccharomyces cerevisiae to elevated concentrations of copper. To understand the basis of this differential resistance, we performed a functional screen for C. albicans genes involved in copper detoxification. Here, we report the isolation of two such genes: a metallothionein, CaCUP1, and a copper-transporting P-type ATPase, CaCRP1. Both genes are induced by extracellular copper. Gene disruptions indicated that the copper extrusion pump is responsible for the unusual resistance of C. albicans to copper, whereas the metallothionein is responsible for the residual copper resistance of the Cacrp1Delta mutant. We show further that under acidic and anaerobic conditions, such as prevail in the natural niche of C. albicans, the digestive tract of animals, CaCRP1 function becomes essential for survival in the presence of even very low copper concentrations. These observations suggest that copper in the gastrointestinal tract may present a toxic challenge to which enteric organisms had to adapt.