Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High‐Molecular‐Weight Proteins

作者：Pierre Gans, Olivier Hamelin, Rémy Sounier, Isabel Ayala, M. Asunción Durá, Carlos Amero, Marjolaine Noirclerc‐Savoye, Bruno Franzetti, Michael J. Plevin, Jérôme Boisbouvier · 发表于：Angewandte Chemie International Edition · 年份：2010 · DOI：10.1002/anie.200905660 · 被引用次数：223 · 研究领域：Protein Structure and Dynamics、RNA and protein synthesis mechanisms、Enzyme Structure and Function

Sometimes less is more: [13C1H3]methyl isotopomers can be biosynthetically incorporated specifically into the pro-S methyl groups of leucine and valine residues in large protein assemblies within a perdeuterated background by using an acetolactate precursor. This stereospecific labeling strategy considerably enhances NMR spectra for large protein assemblies.