A Novel Cytokine Receptor-Ligand Pair

作者：Yanggu Shi, Stephen J. Ullrich, Jun Zhang, Kevin M. Connolly, Krzysztof J. Grzegorzewski, Melisa C. Barber, Wei Wang, Karen Wathen, Vermettya Hodge, Carrie Fisher, Henrik Steen Olsen, Steve M. Ruben, Irina Knyazev, Yun Hee Cho, Viktor Kao, Kirsten A. Wilkinson, Jeffrey A. Carrell, Reinhard Ebner · 发表于：Journal of Biological Chemistry · 年份：2000 · DOI：10.1074/jbc.m910228199 · 被引用次数：237 · 研究领域：Cytokine Signaling Pathways and Interactions、Chemokine receptors and signaling、Monoclonal and Polyclonal Antibodies Research

As part of a large scale effort to discover novel secreted proteins, a cDNA encoding a novel cytokine was identified. Alignments of the sequence of the new protein, designated IL-17B, suggest it to be a homolog of the recently described T cell-derived cytokine, IL-17. By Northern analysis, EST distribution and real-time quantitative polymerase chain reaction analysis, mRNA was detected in many cell types. A novel type I transmembrane protein, identified in an EST data base by homology to IL-17R, was found to bind specifically IL-17B, as determined by surface plasmon resonance analysis, flow cytometry, and co-immunoprecipitation experiments. Readily detectable transcription of IL-17BR was restricted to human kidney, pancreas, liver, brain, and intestines and only a few of the many cell lines tested. By using a rodent ortholog of IL-17BR as a probe, IL-17BR message was found to be drastically up-regulated during intestinal inflammation elicited by indomethacin treatment in rats. In addition, intraperitoneal injection of IL-17B purified from Chinese hamster ovary cells caused marked neutrophil migration in normal mice, in a specific and dose-dependent manner. Together these results suggest that IL-17B may be a novel proinflammatory cytokine acting on a restricted set of target cell types. They also demonstrate the strength of genomic approaches in the unraveling of novel biological pathways.