In Vivo Study of Ligament‐Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold

作者：Jingxian Zhu, Xin Zhang, Zhenxing Shao, Linghui Dai, La Li, Xiaoqing Hu, Xiaokun Wang, Chunyan Zhou, Yingfang Ao · 发表于：Journal of Nanomaterials · 年份：2013 · DOI：10.1155/2013/831873 · 被引用次数：35 · 研究领域：Knee injuries and reconstruction techniques、Silk-based biomaterials and applications、Bone Tissue Engineering Materials

Electrospinning nanofibrous scaffold was commonly used in tissue regeneration recently. Nanofibers with specific topological characteristics were reported to be able to induce osteogenic differentiation of MSCs. In this in vivo study, autologous tendon grafts with lattice‐like nanofibrous scaffold wrapping at two ends of autologous tendon were used to promote early stage of ligament‐bone healing after rabbit ACL reconstruction. To utilize native MSCs from bone marrow, an MSCs specific affinity peptide E7 was conjugated to nanofibrous meshes. After 3 months, H‐E assessment and specific staining of collagen type I, II, and III showed direct ligament‐bone insertion with typical four zones (bone, calcified fibrocartilage, fibrocartilage, and ligament) in bioactive scaffold reconstruction group. Diameters of bone tunnel were smaller in nanofibrous scaffold conjugated E7 peptide group than those in control group. The failure load of substitution complex also indicated a stronger ligament‐bone insertion healing using bioactive scaffold. In conclusion, lattice‐like nanofibrous scaffold with specific MSCs affinity peptide has great potential in promoting early stage of ligament‐bone healing after ACL reconstruction.