Rational Design of “Turn‐On” Allosteric DNAzyme Catalytic Beacons for Aqueous Mercury Ions with Ultrahigh Sensitivity and Selectivity

作者：Juewen Liu, Yi Lu · 发表于：Angewandte Chemie International Edition · 年份：2007 · DOI：10.1002/anie.200702006 · 被引用次数：594 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、Molecular Sensors and Ion Detection

The mercury is rising: A DNAzyme with several thymine residues located close to the catalytic site is rendered inactive. However, thymine–Hg2+–thymine interactions transform the DNAzyme into an active enzyme. When fluorophore/quencher pairs are attached, the DNAzyme can be converted into a highly sensitive and selective mercury sensor with a detection limit of 2.4 nM and a greater than 100 000-fold selectivity over other metal ions.