Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency

作者：Sara Cogliati, Christian Frezza, María Eugenia Soriano, Tatiana Varanita, Rubén Quintana–Cabrera, Mauro Corrado, Sara Cipolat, Veronica Costa, Alberto Casarin, Lígia C. Gomes, Ester Perales‐Clemente, Leonardo Salviati, Patricio Fernández‐Silva, José Antonio Enrı́quez, Luca Scorrano · 发表于：Cell · 年份：2013 · DOI：10.1016/j.cell.2013.08.032 · 被引用次数：1292 · 研究领域：Mitochondrial Function and Pathology、ATP Synthase and ATPases Research、Metabolomics and Mass Spectrometry Studies

Respiratory chain complexes assemble into functional quaternary structures called supercomplexes (RCS) within the folds of the inner mitochondrial membrane, or cristae. Here, we investigate the relationship between respiratory function and mitochondrial ultrastructure and provide evidence that cristae shape determines the assembly and stability of RCS and hence mitochondrial respiratory efficiency. Genetic and apoptotic manipulations of cristae structure affect assembly and activity of RCS in vitro and in vivo, independently of changes to mitochondrial protein synthesis or apoptotic outer mitochondrial membrane permeabilization. We demonstrate that, accordingly, the efficiency of mitochondria-dependent cell growth depends on cristae shape. Thus, RCS assembly emerges as a link between membrane morphology and function.