Soluble cytokine receptors are present in normal human urine.

作者：Daniela Novick, Hartmut Engelmann, David Wallach, Menachem Rubinstein · 发表于：The Journal of Experimental Medicine · 年份：1989 · DOI：10.1084/jem.170.4.1409 · 被引用次数：443 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Atherosclerosis and Cardiovascular Diseases、Immune Cell Function and Interaction

Affinity chromatography of crude human urinary proteins on either human rIL-6, human rIFN-gamma, or anti-IFN-gamma-R mAb yielded the two respective soluble receptors in significant quantities. A single sequence of 30 amino acid residues was obtained by NH2-terminal microsequencing of the protein peak purified in tandem by affinity chromatography on an IL-6 column and reversed-phase HPLC. This sequence was identical to the predicted NH2-terminal sequence of IL-6-R as previously reported. Analysis of the eluted proteins from both IFN-gamma and anti-IFN-gamma-R columns by inhibition of solid phase RIA, ELISA, SDS-PAGE, and Western blotting proved the existence of soluble IFN-gamma-R in normal urine. Our finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.