Mitochondrial DNA content and 4977 bp deletion in unfertilized oocytes

作者：C.C.W. Chan, V.W.S. Liu, Estella Yee Lan Lau, William S.B. Yeung, Ernest Hung Yu Ng, Patrick Ho · 发表于：Molecular Human Reproduction · 年份：2005 · DOI：10.1093/molehr/gah243 · 被引用次数：158 · 研究领域：Mitochondrial Function and Pathology、Metabolism and Genetic Disorders、Birth, Development, and Health

Previous studies analysing the incidences of mitochondrial DNA (mtDNA) deletions and mtDNA content in unfertilized oocytes in relation to donors' age have been controversial. The objective of the study was to compare these two parameters in unfertilized oocytes and relate them to the donors' age. Fifty-two women donated 155 unfertilized metaphase II (MII) oocytes. The incidence of 4977 bp deletion was 34.6%, and the mtDNA copy number was 598 350 +/- 265 862. Women >or=35 years of age had a significantly higher incidence of 4977 bp deletion, lower mtDNA copy number, higher FSH level and poorer ovarian response when compared with younger women. The mtDNA copy number was negatively correlated with the donor's age. The higher incidence of mtDNA deletion and lower mtDNA copy number in older women suggested that these two parameters may reflect ovarian ageing.