The nucleotide sequence of the yeastMEL1gene

作者：Pirkko L. Liljeström · 发表于：Nucleic Acids Research · 年份：1985 · DOI：10.1093/nar/13.20.7257 · 被引用次数：65 · 研究领域：Endoplasmic Reticulum Stress and Disease、Biofuel production and bioconversion、Enzyme Structure and Function

The complete nucleotide sequence of the MEL1 gene of the yeast, Saccharomyces cerevisiae, encoding alpha-galactosidase was determined. The nucleotide sequence contains an open reading frame of 1413 bp encoding a protein of 471 amino acids. Comparison with the known N-terminal amino acid sequence of the mature secreted protein indicated that alpha-galactosidase is synthesized as a precursor with an N-terminal signal sequence of 18 amino acids. The general features of this signal peptide resemble those of other yeast signal peptides. Molecular weight of the mature alpha-galactosidase polypeptide deduced from the nucleotide sequence is 50.049 kd. The 5' regulatory region has sequences in common with other yeast genes regulated by the GAL4-protein.