Improvement of the Representation of Bifidobacteria in Fecal Microbiota Metagenomic Libraries by Application of thecpn60Universal Primer Cocktail

作者：Janet E. Hill, W. M. Ursla Fernando, Gordon Alexander Zello, Robert T. Tyler, Wendy J. Dahl, Andrew G. Van Kessel · 发表于：Applied and Environmental Microbiology · 年份：2010 · DOI：10.1128/aem.01510-09 · 被引用次数：39 · 研究领域：Gut microbiota and health、Genomics and Phylogenetic Studies、Probiotics and Fermented Foods

Actinobacteria, particularly bifidobacteria, are widely observed to be underrepresented in metagenomic studies of microbial communities. We have compared human fecal microbiota clone libraries based on 16S rRNA and cpn60 PCR products. Taxonomic profiles were similar except that the cpn60 libraries contained large numbers of bifidobacterial sequences.