A physical map of the bovine genome

作者：W. M. Snelling, Readman Chiu, Jacqueline E. Schein, Matthew Hobbs, Colette A. Abbey, David L. Adelson, Jan Aerts, Gary L. Bennett, Ian E. Bosdet, Mekki Boussaha, Rüdiger Bräuning, Alexandre Rodrigues Caetano, Marcos Mota do Carmo Costa, Allan M. Crawford, Brian P. Dalrymple, Andre A. Eggen, Annelie Everts-van der Wind, Sandrine Floriot, Mathieu Gautier, C. A. Gill, Ronnie D. Green, Robert A. Holt, O. Jann, Steven J.M. Jones, Steven M. Kappes, J. W. Keele, Pieter J. de Jong, Denis M. Larkin, Harris A. Lewin, John C. McEwan, Stephanie McKay, Marco A. Marra, Carrie Mathewson, Lakshmi K. Matukumalli, S. S. Moore, Brenda M. Murdoch, F. W. Nicholas, Kazutoyo Osoegawa, Alice C. Roy, Hanni Salih, Laurent Schibler, Robert D. Schnabel, Licia Silveri, Loren C. Skow, Timothy P. L. Smith, Tad S. Sonstegard, Jeremy F. Taylor, Ross L. Tellam, Curtis P. Van Tassell, J. L. Williams, James E. Womack, Natasja H. Wye, George Yang, Shaying Zhao · 发表于：Genome biology · 年份：2007 · DOI：10.1186/gb-2007-8-8-r165 · 被引用次数：85 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Genetic and phenotypic traits in livestock、Genomics and Phylogenetic Studies

BACKGROUND: Cattle are important agriculturally and relevant as a model organism. Previously described genetic and radiation hybrid (RH) maps of the bovine genome have been used to identify genomic regions and genes affecting specific traits. Application of these maps to identify influential genetic polymorphisms will be enhanced by integration with each other and with bacterial artificial chromosome (BAC) libraries. The BAC libraries and clone maps are essential for the hybrid clone-by-clone/whole-genome shotgun sequencing approach taken by the bovine genome sequencing project. RESULTS: A bovine BAC map was constructed with HindIII restriction digest fragments of 290,797 BAC clones from animals of three different breeds. Comparative mapping of 422,522 BAC end sequences assisted with BAC map ordering and assembly. Genotypes and pedigree from two genetic maps and marker scores from three whole-genome RH panels were consolidated on a 17,254-marker composite map. Sequence similarity allowed integrating the BAC and composite maps with the bovine draft assembly (Btau3.1), establishing a comprehensive resource describing the bovine genome. Agreement between the marker and BAC maps and the draft assembly is high, although discrepancies exist. The composite and BAC maps are more similar than either is to the draft assembly. CONCLUSION: Further refinement of the maps and greater integration into the genome assembly process may contribute to a high quality assembly. The maps provide re...