Manganese‐guided cellular MRI of human embryonic stem cell and human bone marrow stromal cell viability

作者：Mayumi Yamada, Paul T. Gurney, Jaehoon Chung, Pratima Kundu, Micha Drukker, Alan K. Smith, Irving L. Weissman, Dwight G. Nishimura, Robert C. Robbins, Phillip C. Yang · 发表于：Magnetic Resonance in Medicine · 年份：2009 · DOI：10.1002/mrm.22071 · 被引用次数：29 · 研究领域：Mesenchymal stem cell research、3D Printing in Biomedical Research、Pluripotent Stem Cells Research

This study investigated the ability of MnCl(2) as a cellular MRI contrast agent to determine the in vitro viability of human embryonic stem cells (hESC) and human bone marrow stromal cells (hBMSC). Basic MRI parameters including T(1) and T(2) values of MnCl(2)-labeled hESC and hBMSC were measured and viability signal of manganese (Mn(2+))-labeled cells was validated. Furthermore, the biological activity of Ca(2+)-channels was modulated utilizing both Ca(2+)-channel agonist and antagonist to evaluate concomitant signal changes. Metabolic effects of MnCl(2)-labeling were also assessed using assays for cell viability, proliferation, and apoptosis. Finally, in vivo Mn(2+)-guided MRI of the transplanted hESC was successfully achieved and validated by bioluminescence imaging.