Efficient System for Directed Integration into the Lactobacillus acidophilus and Lactobacillus gasseri Chromosomes via Homologous Recombination

作者：W. M. S. Russell, Todd R. Klaenhammer · 发表于：Applied and Environmental Microbiology · 年份：2001 · DOI：10.1128/aem.67.9.4361-4364.2001 · 被引用次数：149 · 研究领域：Probiotics and Fermented Foods、Genomics and Phylogenetic Studies、Chromosomal and Genetic Variations

An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and Lactobacillus gasseri. The strategy is an adaptation of the lactococcal pORI system (K. Leenhouts, G. Venema, and J. Kok, Methods Cell Sci. 20:35-50, 1998) and relies on the simultaneous use of two plasmids. The functionality of the integration strategy was demonstated by the insertional inactivation of the Lactobacillus acidophilus NCFM lacL gene encoding beta-galactosidase and of the Lactobacillus gasseri ADH gusA gene encoding beta-glucuronidase.