Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes

作者：Timo Hyypiä · 发表于：Journal of Clinical Microbiology · 年份：1985 · DOI：10.1128/jcm.21.5.730-733.1985 · 被引用次数：45 · 研究领域：Virus-based gene therapy research、Respiratory viral infections research、Viral Infections and Immunology Research

The presence of adenovirus DNA in clinical specimens was analyzed by nucleic acid hybridization assays by both radioactive and enzymatic detection systems. The sensitivity of the hybridization tests was in the range of 10 to 100 pg of homologous adenovirus DNA. Minimal background was noticed with unrelated viral and nonviral DNA. Twenty-four nasopharyngeal mucus aspirate specimens, collected from children with acute respiratory infection, were assayed in the hybridization tests and also by an enzyme immunoassay for adenovirus hexon antigen which was used as a reference test. Sixteen specimens positive by the enzyme immunoassay also were positive in the two nucleic acid hybridization tests, and the remaining eight specimens were negative in all of the tests. The results indicate that nucleid acid hybridization tests with both radioactive and nonradioactive probes can be used for diagnosis of microbial infections.