Response of Brassica napus in tissue culture to metsulfuron‐methyl and chlorsulfuron

作者：SEIDEN, Kappel, Jens Carl Streibig · 发表于：Weed Research · 年份：1998 · DOI：10.1046/j.1365-3180.1998.00091.x · 被引用次数：12 · 研究领域：Weed Control and Herbicide Applications、Pesticide and Herbicide Environmental Studies、Plant tissue culture and regeneration

A herbicide bioassay based on tissue cultures ofBrassica napusL. was evaluated with selected sulfonylurea herbicides. Data were analysed by fitting the results to a log‐logistic dose–response model. Within an experiment, the non‐linear regression models were fitted simultaneously to the individual dose–response curves. The results obtained showed good response to even low concentrations of herbicide, with detection limits in the range 0.008–0.69 nmol L−1for chlorsulfuron and 0.02–0.13 nmol L−1for metsulfuron. The reproducibility of the assays, on the basis of coefficient of variation of theED50values, was found to be 44% for chlorsulfuron and 48% for metsulfuron measurements. Assay of herbicide dissolved in aqueous soil extract showed significant interference from this matrix on the response, requiring a five times dilution of the extract to overcome this matrix effect.