Expression of interleukin 8 correlates with the metastatic potential of human melanoma cells in nude mice.

作者：Rakesh K. Singh, Mordechai Gutman, Robert Radinsky, CORAZON D. BUCANA, Isaiah J. Fidler · 发表于：PubMed · 年份：1994 · 被引用次数：364 · 研究领域：Immunotherapy and Immune Responses、Virus-based gene therapy research、Cancer Cells and Metastasis

We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.