Cloning and characterization of a human type II receptor for bone morphogenetic proteins.

作者：Bradley Rosenzweig, Takeshi Imamura, Toshihide Okadome, George N. Cox, Hidetoshi Yamashita, Peter ten Dijke, Carl‐Henrik Heldin, Kohei Miyazono · 发表于：Proceedings of the National Academy of Sciences · 年份：1995 · DOI：10.1073/pnas.92.17.7632 · 被引用次数：551 · 研究领域：TGF-β signaling in diseases、Connective Tissue Growth Factor Research、Connective tissue disorders research

Bone morphogenetic proteins (BMPs) are members of the transforming growth factor beta superfamily. Several members of this family have been shown to transduce their signals through binding to type I and type II serine-(threonine) kinase receptors. Here we report the cDNA cloning and characterization of a human type II receptor for BMPs (BMPR-II), which is distantly related to DAF-4, a BMP type II receptor from Caenorhabditis elegans. In transfected COS-1 cells, osteogenic protein (OP)-1/BMP-7, and less efficiently BMP-4, bound to BMPR-II. BMPR-II bound ligands only weakly alone, but the binding was facilitated by the presence of previously identified type I receptors for BMPs. Binding of OP-1/BMP-7 to BMPR-II was also observed in nontransfected cell lines. Moreover, a transcriptional activation signal was transduced by BMPR-II in the presence of type I receptors after stimulation by OP-1/BMP-7.