Enhanced Mechanical Stability of Microtubules Polymerized with a Slowly Hydrolyzable Nucleotide Analogue

作者：Kathleen M. Munson, Philipose G. Mulugeta, Zachary J. Donhauser · 发表于：The Journal of Physical Chemistry B · 年份：2007 · DOI：10.1021/jp0716637 · 被引用次数：24 · 研究领域：Microtubule and mitosis dynamics、Cellular Mechanics and Interactions、Nuclear Structure and Function

Atomic force microscopy (AFM) has been used to investigate the local mechanical and structural properties of microtubules polymerized using guanylyl-alpha-beta-methylene diphosphonate (GMPCPP), a slowly hydrolyzable analogue of guanosine triphosphate. Using a combination of AFM imaging and local force spectroscopy, GMPCPP-polymerized microtubules have been qualitatively and quantitatively compared to paclitaxel-stabilized microtubules. GMPCPP-polymerized microtubules qualitatively display a greater resistance to destruction by the AFM probe tip during imaging and during deformation measurements and maintain structural details after indentation. In addition, using force spectroscopy taken during the indentation and collapse of individual microtubules with the AFM probe tip, an effective spring constant of the microtubule wall (kMT) for both types of microtubules was determined. The average kMT of GMPCPP-polymerized microtubules, 0.172 N/m, is more than twice that of paclitaxel-stabilized microtubules. These results complement previously reported measurements of bending experiments on GMPCPP-polymerized and paclitaxel-stabilized microtubules.