Silver Impregnation of Degenerating Axons in the Central Nervous System: A Modified Technic

作者：W. J. H. Nauta, P. A. Gygax · 发表于：Stain Technology · 年份：1954 · DOI：10.3109/10520295409115448 · 被引用次数：825 · 研究领域：Bone Tissue Engineering Materials、3D Printing in Biomedical Research、Nerve injury and regeneration

Frozen sections of formalin-fixed brains containing surgical lesions, were treated with 15% ethanol for 0.5 hr., soaked in 0.5% phosphomolybdic acid for 0.25–1.0 hr., and subsequently treated with 0.05% potassium permanganate for 4–10 min. (The duration of the latter treatment is critical and individually variable). Subsequent procedure is as follows: decolorize in a mixture of equal parts of 1% hydroquinone and 1% oxalic acid; wash thoroughly and soak sections in 1.5% silver nitrate for 20–30 min.; ammoniacal silver nitrate (silver nitrate 0.9 g., distilled water 20 ml., pure ethanol 10 ml., strong ammonia 1.8 ml., 2.5% sodium hydroxide 1.5 ml.) 0.5–1.0 min.; reduce in acidified formalin (distilled water 400 ml., pure ethanol 45 ml., 1% citric acid 13.5 ml., 10% formalin 13.5 ml.) 1 min.; wash, and pass section through 1 % sodium thiosulf ate (0.5–1.0 min.); wash thoroughly and pass sections through graded alcohols and xylene (3 changes); cover in neutral synthetic resin.