MEMBRANE SPLITTING IN FREEZE-ETCHING

作者：Pedro Pinto da Silva, Daniel Branton · 发表于：The Journal of Cell Biology · 年份：1970 · DOI：10.1083/jcb.45.3.598 · 被引用次数：568 · 研究领域：Protein purification and stability、Lipid Membrane Structure and Behavior、Nanopore and Nanochannel Transport Studies

The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching.