CROSS REACTION IN REVERSED PASSIVE HEMAGGLUTINATION BETWEEN CLOSTRIDIUM BOTULINUM TYPE A AND B TOXINS AND ITS AVOIDANCE BY THE USE OF ANTITOXIC COMPONENT IMMUNOGLOBULIN ISOLATED BY AFFINITY CHROMATOGRAPHY

作者：Genji Sakaguchi, Sumiko Sakaguchi, Shunji Kozaki, Shunji Sugii, Iwao Ohishi · 发表于：Japanese Journal of Medical Science and Biology · 年份：1974 · DOI：10.7883/yoken1952.27.161 · 被引用次数：43 · 研究领域：Botulinum Toxin and Related Neurological Disorders、Clostridium difficile and Clostridium perfringens research、Streptococcal Infections and Treatments

Passive hemagglutination and agar gel double diffusion tests demonstrated cross reactions between Clostridium botulinum type A and B toxins to a high level and between types E and F and types A and F to a much lower level. The cross reactions between types A and B and types A and F were due to the nontoxic component; that between types E and F to both the toxic and nontoxic components.Anti-toxic component immunoglobulins types A and B were isolated from anti-progenitor toxin of the corresponding types by affinity chromatography. Reversed passive hemagglutination tests with formalinized sheep red blood cells coupled with anti-toxic component immunoglobulins type A or B caused no cross reaction between these types and detected 8-89 LD50/ml of type A and 10-59 LD50/ml of type B toxins in either a crude or purified form.Direct hemagglutinin possessed by type A and some type B toxins did not disturb the reversed passive hemagglutination tests.