Microfluidic Synthesis of Rigid Nanovesicles for Hydrophilic Reagents Delivery

作者：Lu Zhang, Qiang Feng, Jiuling Wang, Jiashu Sun, Xinghua Shi, Xingyu Jiang · 发表于：Angewandte Chemie International Edition · 年份：2015 · DOI：10.1002/anie.201500096 · 被引用次数：152 · 研究领域：Microfluidic and Capillary Electrophoresis Applications、Innovative Microfluidic and Catalytic Techniques Innovation、Microfluidic and Bio-sensing Technologies

We present a hollow-structured rigid nanovesicle (RNV) fabricated by a multi-stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic-co-glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) simulations. The entrapment efficiency of hydrophilic reagents such as calcein, rhodamine B and siRNA inside the hollow water core of RNV is ≈90 %. In comparison with the combination of free Dox and siRNA, RNV that co-encapsulate siRNA and doxorubicin (Dox) reveals a significantly enhanced anti-tumor effect for a multi-drug resistant tumor model.