Distribution of molecular species of sphingomyelins in different parts of bovine digestive tract

作者：Michael E. Breimer · 发表于：Journal of Lipid Research · 年份：1975 · DOI：10.1016/s0022-2275(20)36725-0 · 被引用次数：27 · 研究领域：Sphingolipid Metabolism and Signaling、Lipid Membrane Structure and Behavior、Caveolin-1 and cellular processes

Sphingomyelins were isolated from mucosal layers of bovine rennet stomach, duodenum, jejunoileum, and colon ascendens.The mramides obtained after phospholipase degradation were characterized by thin-layer chromatography, mass spectrometry, and gas-liquid chromatography.The main ceramide group from all regions consisted of dihydroxy long-chain bases and normal fatty acids.Sphingosine was the predominant base in all these fractions, and only in rennet stomach were smaller amounts of the Cl7 and CZO homologs present.Normal saturated C l 6 , CIS, (222, and C24 fatty acids were most abundant.In rennet stomach there was in addition a ceramide group having dihydroxy long-chain bases in combination with hydroxy fatty acids.Sphingosine was the predominant long-chain base and the fatty acids were 2-hydroxy C I S , C22, C23, and C24.From jejunoileum three minor ceramide fractions were isolated; these consisted of phytosphingosine and normal fatty acids C2 2-c~ 4), sphingosine and 2-hydroxy fatty acids (C 4), and phytosphingosine and 2-hydroxy fatty acids ( C Z ~-C Z ~) , respectively.No branched paraffin chains were found in significant amounts.Sphingomyelins with trihydroxy long-chain bases and 2-hydroxy fatty acids found in jejunoileum were also detected in bovine kidney and have not been demonstrated before.These sphingomyelins from both kidney and jejunoileum showed a preferential combination of trihydroxy bases and fatty acids with very long chains ( C Z Z -C ~~) .Supplementary key...