Neutrophil Recruitment and Resistance to Urinary Tract Infection

作者：Masashi Haraoka, Long Hang, Björn Frendéus, Gabriela Godaly, Marie D. Burdick, Robert M. Strieter, Catharina Svanborg · 发表于：The Journal of Infectious Diseases · 年份：1999 · DOI：10.1086/315006 · 被引用次数：274 · 研究领域：Immune Response and Inflammation、Urinary Tract Infections Management、Immune cells in cancer

This study examined the role of neutrophil leukocytes for the antibacterial defense at mucosal infection sites. Urinary tract infection (UTI) was established by injection into the bladder lumen of Escherichia coli 1177, a fully virulent clinical isolate. Infection of C3H/HeN (lpsn, lpsn) mice recruited neutrophils into the urinary tract, and bacteria were cleared from kidneys and bladders. The neutrophil response was absent in C3H/HeJ (lpsd, lpsd) mice, and bacteria persisted in the tissues. Peripheral neutrophil depletion of C3H/HeN mice was subsequently achieved by pretreatment with the granulocyte-specific antibody RB6-8C5. The E. coli-induced neutrophil recruitment was inhibited, as shown by immunohistochemistry and tissue myeloperoxidase quantitation. As a consequence, bacterial clearance from kidneys and bladders was drastically impaired. Antibody treatment of C3H/HeJ mice had only a marginal effect. The results show that neutrophils are essential for bacterial clearance from the urinary tract and that the neutrophil recruitment deficiency in C3H/HeJ mice explains their susceptibility to gram-negative UTI.