High-frequency shoot regeneration of nodal explants from Tetrastigma hemsleyanum Diels et Gilg: A valuable medicinal plant

作者：Weimei Jiang, Yuanmiao Fu, Xiaolong Zhou, Chengxin Fu · 发表于：AFRICAN JOURNAL OF BIOTECHNOLOGY · 年份：2011 · 被引用次数：7 · 研究领域：Plant tissue culture and regeneration、Plant Pathogens and Fungal Diseases、Seed Germination and Physiology

This paper describes the shoot regeneration of nodal segments from a medicinal plant, Tetrastigma hemsleyanum Diels et Gilg (Vitaceae). The highest number of shoots (7.27 shoots per explant) was observed in MS medium supplemented with 4 mg/l BA after six weeks of inoculation. 2 mg/l BA in combination with 0.1 mg/l NAA not only induced shoot proliferation but also increased shoot length. Well-developed shoots were rooted on half strength MS medium supplemented with 2 mg/l IBA with 100% rooting and 85% of the regenerated plantlets survived before been transferred to field conditions. Key words: Medicinal plant, nodal explants, shoot regeneration, Tetrastigma hemsleyanum.