Detecting Protein Function and Protein-Protein Interactions from Genome Sequences

作者：Edward M. Marcotte, Matteo A. Pellegrini, Ho Leung Ng, Danny W. Rice, Todd O. Yeates, David S Eisenberg · 发表于：Science · 年份：1999 · DOI：10.1126/science.285.5428.751 · 被引用次数：1689 · 研究领域：RNA and protein synthesis mechanisms、Bioinformatics and Genomic Networks、Protein Structure and Dynamics

A computational method is proposed for inferring protein interactions from genome sequences on the basis of the observation that some pairs of interacting proteins have homologs in another organism fused into a single protein chain. Searching sequences from many genomes revealed 6809 such putative protein-protein interactions in Escherichia coli and 45,502 in yeast. Many members of these pairs were confirmed as functionally related; computational filtering further enriches for interactions. Some proteins have links to several other proteins; these coupled links appear to represent functional interactions such as complexes or pathways. Experimentally confirmed interacting pairs are documented in a Database of Interacting Proteins.