Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects.

作者：A. Barry Kay, S Ying, Veronica Varney, Mina Gaga, Stephen R. Durham, Redwan Moqbel, Andrew John Wardlaw, Qutayba A. Hamid · 发表于：The Journal of Experimental Medicine · 年份：1991 · DOI：10.1084/jem.173.3.775 · 被引用次数：671 · 研究领域：Eosinophilic Disorders and Syndromes、IL-33, ST2, and ILC Pathways、Urticaria and Related Conditions

Cryostat sections from skin biopsies from 24-h allergen-induced late-phase cutaneous reactions (LPR) in 14 human atopic subjects were hybridized with 35S-labeled RNA probes for a number of cytokines. mRNA was detected for interleukin 3 (IL-3) (8/14), IL-4 (10/14), IL-5 (11/14), and granulocyte/macrophage colony-stimulating factor (GM-CSF) (13/14). Only 5 of 14 gave hybridization signals for IL-2, and 0 of 14 for interferon gamma. Biopsies from diluent controls gave only occasional weak signals. These results suggest that cells infiltrating the site of the 24-h LPR transcribe mRNA for the IL-3, IL-4, IL-5, and GM-CSF gene cluster and support the hypothesis that atopy is associated with preferential activation of cells having a similar cytokine profile to the murine T helper type 2 subset.