Phospholipid supplementation reverses behavioral and biochemical alterations induced by n–3 polyunsaturated fatty acid deficiency in mice

作者：Isabelle Carrié, Michel Clément, Dominique de Javel, Henriette Francès, Jean‐Marie Bourre · 发表于：Journal of Lipid Research · 年份：2000 · DOI：10.1016/s0022-2275(20)34486-2 · 被引用次数：163 · 研究领域：Fatty Acid Research and Health、Biochemical Analysis and Sensing Techniques、Animal Nutrition and Physiology

This study investigated the effects of a diet deficient in alpha-linolenic acid followed or not by supplementation with phospholipids rich in n-3 polyunsaturated fatty acids (PUFA) on behavior and phospholipid fatty acid composition in selected brain regions. Three weeks before mating, two groups of mice were fed a semisynthetic diet containing both linoleic and alpha-linolenic acid or a diet deficient in alpha-linolenic acid. Pups were fed the same diet as their dams. At the age of 7 weeks, a part of the deficient group was supplemented with n-3 PUFA from either egg yolk or pig brain phospholipids for 2 months. In the open field, rearing activity was significantly reduced in the deficient group. In the elevated plus maze (anxiety protocol), the time spent on open arms was significantly smaller in deficient mice than in controls. Using the learning protocol with the same task, the alpha-linolenic acid deficiency induced a learning deficit. Rearing activity and learning deficits were completely restored by supplementation with egg yolk or cerebral phospholipids, though the level of anxiety remained significantly higher than that of controls. There were no differences among the 4 diet groups for either the Morris water maze or passive avoidance. In control mice, the level of 22:6 n-3 was significantly higher in the frontal cortex compared to all other regions analysed. The frontal cortex and the striatum were the most markedly affected by the deficiency. Supplementation with ph...