DNA Immunization for Influenza Virus: Studies Using Hemagglutinin‐ and Nucleoprotein‐Expressing DNAs

作者：Harriet L. Robinson, Christine Boyle, David Feltquate, Merribeth J. Morin, Joseph C. Santoro, Robert G. Webster · 发表于：The Journal of Infectious Diseases · 年份：1997 · DOI：10.1086/514176 · 被引用次数：113 · 研究领域：Influenza Virus Research Studies、Immunotherapy and Immune Responses、Respiratory viral infections research

DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.