Synthetic filaments assembled from C‐terminally truncated α‐synuclein

作者：R. Anthony Crowther, Ross Jakes, Maria Grazia Spillantini, Michel Goedert · 发表于：FEBS Letters · 年份：1998 · DOI：10.1016/s0014-5793(98)01146-6 · 被引用次数：423 · 研究领域：Parkinson's Disease Mechanisms and Treatments、Neurological disorders and treatments、Neurological diseases and metabolism

Recently two point mutations in the alpha-synuclein gene have been found in familial Parkinson's disease. The characteristic fibrous neuropathological lesions of Parkinson's and other neurodegenerative diseases have been shown to stain strongly with antibodies against alpha-synuclein and extracted filaments have been labelled with anti-alpha-synuclein antibodies. In view of the close involvement of alpha-synuclein filaments with pathology, it was important to establish an in vitro assembly system. We report here that C-terminally truncated recombinant alpha-synuclein readily assembles into filaments resembling those isolated from diseased brain and suggest that truncation by proteolysis may play a role in the pathological process.