Sexing chick embryos: A rapid and simple protocol

作者：Michael Clinton, L. Haines, B. Belloir, Derek McBride · 发表于：British Poultry Science · 年份：2001 · DOI：10.1080/713655025 · 被引用次数：151 · 研究领域：Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、Genetic and phenotypic traits in livestock、Animal Genetics and Reproduction

1. Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development. 2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming. 3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.