FINE-STRUCTURE MAPPING OF THE ACETAMIDASE STRUCTURAL GENE AND ITS CONTROLLING REGION IN ASPERGILLUS NIDULANS

作者：Michael J. Hynes · 发表于：Genetics · 年份：1979 · DOI：10.1093/genetics/91.3.381 · 被引用次数：42 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Enzyme Catalysis and Immobilization、Algal biology and biofuel production

A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not produce detectable inactive gene product, was found to be inseparable by recombination from the "up-promoter" mutation amdI18 and was located outside of the apparent amdS coding region. The cis-dominant mutation, amdI9, was also located at this end of the gene. This work, therefore, provides evidence for the separation of a eukaryotic gene into controlling and structural regions.