Identifying tumor stem‐like cells in mouse melanoma cell lines by analyzing the characteristics of side population cells

作者：Jun Dou, Ping Wen, Weihua Hu, Yating Li, Yun Wu, Chunsheng Liu, Fengshu Zhao, Kai Hu, Jing Wang, Chuilian Jiang, Xiangfeng He, Ning Gu · 发表于：Cell Biology International · 年份：2009 · DOI：10.1016/j.cellbi.2009.05.003 · 被引用次数：36 · 研究领域：Cancer Cells and Metastasis、Renal and related cancers、Epigenetics and DNA Methylation

Increasingly more evidence shows that TSCs possess the characteristics of stem-like cells. However, a link between stem cells and TSCs remains to be shown. We have sorted SP cells and non-SP cells from the B16F10 cell lines by FACS, and then studied their cellular biological characteristics by using a SFCM culture method, proliferative assay in vitro, clone formation assays in soft agar and normal media, tumorigenic assays in C57BL/6 mice, and resistance to chemotherapy assay in vitro, the quantitative detecting expression of ABCG2 and their CD phenotype analysis by a FCM. We detected 0.96% SP cells in the B16F10 cells and found that they had obvious differences in characteristics from non-SP cells. They possessed a marked capacity for self-renewal in soft agar and culture medium, strong tumorigenic potential in C57BL/6 mice, apparent resistance to vinblastin in vitro, upregulated ABCG2 expression, and a high expression of CD44+CD133+CD24+ phenotypes. We conclude that there were a few of SP cells that had the characteristics of tumor stem-like cells which may provide a useful tool and a readily accessible source for further study when specific TSCs markers are unknown.